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Oral Abstracts (Session 3 of 5)
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(Presentation 34.1) Lactoferrin promotes human skin keratinocyte proliferation Juan Chen, MD; James J. Wu; Jie Li, MD, PhD, University of Miami, Miami, Fla Lactoferrin, a member of the transferrin family of iron-binding protein, has been shown to promote growth of several cell lines from non-skin systems in addition to its known antimicrobial activities. However, its function as a potential growth factor in wound healing has not been investigated. The objective of this study was to evaluate the effects of lactoferrin on skin keratinocyte growth, which is critical to wound repair after injury. The authors conducted an in-vitro 7-day timing course of the keratinocyte proliferation analysis with a preparation of iron-bound recombinant human lactoferrin (holo-rhLF) from a transgenic rice plant. Normal human skin keratinocytes were grown in EpiLife base medium with human keratinocyte growth supplements (HKGS) without transferrin but with holo-rhLF added at concentrations of 0 (LF0), 10 (LF10), and 100 (LF100) ug/mL. The medium with complete HKGS was used as a positive control. Significant increases in keratinocyte proliferation were found in the medium with LF added at the low concentration of 10 ug/mL at Day 5 and Day 6 compared with the medium without LF (LF0) (P < 0.01). More strikingly, strong stimulatory effects of LF on keratinocyte proliferation were observed at the concentration of 100 ug/mL at Days 5–7 (P < 0.001) compared with control and other test groups. This study indicates that rhLF may have an important function in wound healing by promoting keratinocyte proliferation. References Huang N. High-level protein expression system uses self-pollinating crops as host. BioProcess Int. 2004;2(1):54–59. Lonnerdal B, Iyer S. Lactoferrin: molecular structure and biological function. Ann Rev Nutr. 1995;15:93–110. Naot D, Grey A, Reid IR, Cornish J. Lactoferrin—a novel bone growth factor. Clin Med Res. 2005;3:93–101. Nichols BL, McKee K, Putman M, Henry JF, Nichols VN. Human lactoferrin supplementation of infant formulas increases thymidine incorporation into the DNA of rat crypt cells. J Pediatr Gastroenterol Nutr. 1989;8:102–129. Ward PP, Uribe-Luna S, Conneely OM. Lactoferrin and host defense. Biochem Cell Biol. 2003;80: 95–102. |
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