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Oral Abstracts (Session 1 of 5)
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Moderator: Patricia M. Mertz, BA (Presentation 32.6) The effect of a topical oxygen emulsion on collagen deposition and angiogenesis in partial-thickness wounds Jie Li, MD, PhD; Linjian Zhu, MS; Kiral Rahill; Ya-Xiong Tang, PhD; Ramon B. Montero, BS; Patricia M. Mertz, BS; William H. Eaglstein, MD; Stephen C. Davis, BS, University of Miami, Miami, Fla; Paul Zalesky, PhD; Li-Chien Hsu, PhD; Jeff Creech, PhD, TherOx Inc., Irvine, Calif Abstract: A porcine partial-thickness wound model was used to evaluate the effects of a newly developed topical oxygen emulsion (TOE) on dermal wound repair. The wounds were treated with air exposure, vehicle control, or TOE (which contains super-saturated oxygen). Wound samples from 6 pigs were collected at Days 0, 1, 4, 7, 10, 14, and 21 after wounding. Semi-quantitative immunofluorescent staining and RT-PCR (Reverse Transcription and Polymerase Chain Reaction) were performed to examine protein production and mRNA expression for type I and type III collagens and vascular endothelial growth factor (VEGF). Immunofluorescent staining revealed higher protein levels of collagen I, collagen III, and VEGF in the AOE treatment group. A significant increase of collagen III protein occurred earlier with a peak at Day 3 through Day 7, while higher collagen I production was detected later with peak at Days 7, 14, and 21. Significantly higher expression of VEGF was observed as early as Day 1 and through Day 7. RT-PCR analysis result is consistent with the data from the immunofluorescent staining. The study suggests that sustained high level of oxygen released by TOE may promote the process of wound repair through the mechanism of increased collagen deposition and angiogenesis. |
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