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Medicine: Evidence-Based Research: Plenary Session
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Moderator: George T. Rodeheaver, PhD Abstract This session will present new evidence-based data from recently performed clinical trails to be chosen before the meeting. Dr. Rodeheaver will moderate the session and provide introductions where appropriate. (Presentation 23.2) Bioactive small intestinal submucosa stimulates VEGF secretion Jason P. Hodde, MS; Abram D. Janis, MS, Cook Biotech Incorporated, West Lafayette, Ind Abstract: Bioactive materials have been effective in treating chronic wounds, shifting the static wound environment to a more active acute phase. Small intestinal submucosa (SIS*) is an extracellular matrix (ECM)-derived biomaterial for the treatment of soft tissue injury, including pressure, venous, and diabetic ulcers. The collagen-based SIS also contains other proteins, including growth factors involved at various stages of wound healing. Ethylene oxide (EO)-sterilized SIS* has been shown to contain basic fibroblast growth factor (FGF-2), transforming growth factor (TGF), and connective tissue growth factor (CTGF) in nanogram quantities, yet vascular endothelial growth factor (VEGF) is present only in the picogram range. Vascular endothelial growth factor is loosely bound in the ECM and is extremely susceptible to degradation by EO gas. Recent experiments demonstrate the ability of EO-sterilized SIS to stimulate fibroblast production of VEGF in vitro. This response was significantly reduced when an activity-neutralizing antibody specific for FGF-2 was added to the conditioned cell culture medium. These results demonstrate the ability of SIS to stimulate cells to cause a downstream response. This upregulation of endogenous growth factor is a newly discovered potential mechanism that may explain the improvement in wound healing during treatment with SIS. * Oasis® Wound Matrix (Cook Biotech Incorporated, distributed exclusively by Healthpoint, Fort Worth, Tex) References 1. Demling RH, Niezgoda JA, Haraway GD, Mostow EN. Small intestinal submucosa wound matrix and full-thickness venous ulcers: preliminary results. WOUNDS. 2004;16:18–22. 2. Hodde JP, Hiles MC. Bioactive FGF-2 in sterilized extracellular matrix. WOUNDS. 2001;13:195–201. |
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