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Lab Research
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Epidermal Growth Factor Film Formulation for Advanced Wound Treatment First Author: Sunhee Kim Authors: Sangkil Lee, Yunwha Kim, Chaeha Yoon, Sunmi Yang, Sanghyun Nam, Kyungsun Shin, JoonPio Hong, Seunggook Park Wound healing goes through very complex process. In wound healing process, physical and biochemical aspects such as moist condition of wound site, action of growth factors and immune cells are considered as important factors. More specifically, growth factors control the growth, differentiation and metabolism of cells and regulate the process of tissue repair. In this research, we developed recombinant human epidermal growth factor (rhEGF) containing film formulation and evaluated the wound healing effect. The rhEGF-loaded film was formulated with combination of biopolymers and prepared by casting and drying technique. The physical characteristics (i.e. thickness, moist contents and morphology) were measured and observed, and short-term stability in preparation process and room temperature was performed. The in vitro release test and the wound healing effect were estimated by comparing with two controls of ointment and spray formulation with full-thickness pig model. Finally, we compared the anti-bacterial effect of film formulation with commercial silver product with 4 representative bacteria of S. aureus, P. aeruginosa, MRSA and VRE. The final film tested has 120±5.0? of mean thickness and about 20% of moist contents. The bioactivity and content of rhEGF were fully maintained almost 100% after manufacturing process, and the stability of rhEGF was maintained over 1 month at room temperature. In vitro release test revealed that the film formulation released rhEGF as a first-order fashion for 1 day and the rate of granulation and re-epithelialization were shortened compared with control formulations. The present film formulation significantly decreased growth rate of all bacteria and the anti-bacterial effect was similar with that of silver releasing product. The advanced wound healing effect of film formulation might arise from the rhEGF sustained releasing characteristics. The sustained rhEGF release characteristics and anti-bacterial effect can give convenience to nurses and doctors in patient care, and reduce the labor cost of them. Moreover, the one-day rhEGF releasing film formulation is also able to reduce the patient's economic burden and increase quality of life by giving enhanced healing results. In conclusion, rhEGF film was considered as a pharmacoeconomically meaningful and safety-guaranteed wound-healing product candidate. |
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