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Lab Research
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Stimulation of human keratinocyte and fibroblasts cells with a new hypochlorous acid antiseptic First Author: George Cherry Authors: Margaret Hughes, Joseph Selkon, Chufa He We previously reported enhanced healing of patients with venous leg ulcers treated with a new hypochlorous acid antiseptic agent. and demonstrated a significant reduction of the bacterial load after treatment The objective of this study was to determine whether hypochlorous acid was toxic to human cells in vitro and what was the optimal pH for its use. Method Hypochlorous acid solutions was prepared at pH 4.2, 5.4 and 6.4. Cells were seeded in plates, cultured for two days and then exposed to various dilutions of hypochlorous acid in buffered saline for 15 to 60 minutes. This was then washed off and replaced with culture medium. After 3,, 5 or 6 days the cells were assayed by a colorimetric method (MTT). Results: Initial experiments on fibroblasts showed that hypochlorous acid at pH 5.4 was the best for cell growth. Inhibition of fibroblasts in this solution was observed at concentrations greater than 1 in 20. However, in more dilute concentrations from 1/4000 to 1/20 there was significant stimulation by day 6 to 140%-180% of controls. No toxicity to keratinocytes was observed at concentrations up to 1 in 7. Concentrations of hypochlorous acid from 1/20 to 1/2000 significantly stimulated the growth of keratinocytes by day 5, up to 135% of controls. Conclusion The cell culture studies appear to back up the positive clinical effects of this agent and the solution at pH 5.4 was recommended for clinical use. The results of a major clinical study were presented last year. * Product notation: *Steriloxª, Puricore, 320 King of Prussia Rd, Rednor, Pa 19087 USA |
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