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Lab Research
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effects of wound care dressings on cellular proliferation and fibroblast adherence assessed in vitro First Author: François-Xavier BERNARD Authors: Bernard F.X., Barrault C., Bohbot S Introduction: A preliminary in vitro study demonstrated that contact between normal human fibroblasts and a novel wound dressing* induced fibroblast proliferation compared to contact with paraffin gauze or non-adherent dressings. A second in vitro study was conducted to confirm these results comparing this dressing with other non adherent dressings with respect to adherence and proliferation of normal human dermal fibroblasts (NHDF). Materials and methods: NHDF were cultivated in Dulbecco's Minimal Essential Medium (DMEM) supplemented with 10% foetal calf serum in 12-well plates. Samples of five different wound dressings were applied directly to the cultures and allowed to remain for various periods of time. Cell proliferation was measured via [3H] thymidine incorporation. Results: Of the five tested dressings, only the novel hydrocolloid technology induced a stimulation of NHDF proliferation. Two other dressings had no effect and two others had a cytostatic effect. In addition, the cell lesions induced by dressing removal were more severe with four other dressings than the tested dressing, due to lower adherence to the cell surface and/or extracellular matrix. Conclusion: The use of the novel wound dressing stimulates the fibroblast proliferation which may be beneficial in practice. However, clinical evaluations are required to confirm these in vitro results. *Restore Contact Layer with Triact Technology, Hollister Wound Care, Libertyville, IL Supported in part by Hollister Wound Care, LLC |
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