2005 Symposium on Advanced Wound Care | |
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Laboratory Research
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Evaluation of the antimicrobial properties and silver release profile of an antimicrobial silver alginate wound dressing Deborah Addison, BSC(Hons), CChem, MRSC, Tracy J Rennison BSc(Hons), Michelle Del Bono, Johnson & Johnson Wound Management, Gargrave, UK Simulated in-use tests were developed to determine the silver ion release and the antimicrobial properties of an antimicrobial silver alginate dressing. Two test methods were developed: 1) Atomic absorption to determine silver ion release in simulated wound fluid, 2) A log reduction test that exposes a small sample of dressing against a high bacterial loaded broth, measuring the log reduction of the bacteria over a specified time period. The dressing was placed and agitated in simulated wound fluid at 370C. Each day the sample was transferred to fresh wound fluid. At each 24-hour time point, the silver ion concentration in the simulated wound fluid was determined by atomic absorption. At the end of the extended test period the same dressing sample was then placed in a log reduction test. The results shows that in simulated prolonged use conditions that the silver ion release for the product is sustained and consistent. The study is still in progression but close to completion. The antimicrobial alginate dressing in simulated in-use tests has demonstrated sustained and consistent silver ion release in simulated wound fluid over a specified time period. The log reduction in-vitro microbiology testing that followed the simulated in-use test shows that the dressing is effective at reducing the number of viable bacteria within the broth. |
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